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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6255 | 3' | -57.5 | NC_001844.1 | + | 117623 | 0.67 | 0.787087 |
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Target: 5'- cACCC-CAgcuggGaGAGGCCAUgGCCCGCGUu -3' miRNA: 3'- -UGGGuGUa----C-CUCCGGUG-UGGGUGUAc -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 142429 | 0.67 | 0.758943 |
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Target: 5'- gGCCCGag-GGccGCgCACACCCugGUGu -3' miRNA: 3'- -UGGGUguaCCucCG-GUGUGGGugUAC- -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 41518 | 0.68 | 0.729785 |
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Target: 5'- uUCCAgGUGGGGGCgGCuACCgGCAg- -3' miRNA: 3'- uGGGUgUACCUCCGgUG-UGGgUGUac -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 14057 | 0.68 | 0.718888 |
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Target: 5'- gGCUCGCGUGGAGGCgccaagaUACuuCCCugGUa -3' miRNA: 3'- -UGGGUGUACCUCCG-------GUGu-GGGugUAc -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 57102 | 0.68 | 0.709906 |
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Target: 5'- cGCCU--GUGGAGGCCGagcagguGCCgGCAUGa -3' miRNA: 3'- -UGGGugUACCUCCGGUg------UGGgUGUAC- -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 113749 | 0.69 | 0.678596 |
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Target: 5'- gGCCUACAcggUGGAcacccgGGCCggagagcGCACCCGCGUu -3' miRNA: 3'- -UGGGUGU---ACCU------CCGG-------UGUGGGUGUAc -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 114592 | 0.69 | 0.659207 |
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Target: 5'- uCCC-CAUGGAGacuGCCACACgCCACc-- -3' miRNA: 3'- uGGGuGUACCUC---CGGUGUG-GGUGuac -5' |
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| 6255 | 3' | -57.5 | NC_001844.1 | + | 17602 | 0.77 | 0.251921 |
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Target: 5'- cGCCUccagcGCAUGGcGGCUACGCCCACAc- -3' miRNA: 3'- -UGGG-----UGUACCuCCGGUGUGGGUGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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