Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6256 | 3' | -59.4 | NC_001844.1 | + | 22783 | 0.66 | 0.752157 |
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Target: 5'- --uGGCCGCaACCCACAUuucaaGGuGGCUg- -3' miRNA: 3'- ccuCCGGUG-UGGGUGUA-----CCuCCGGug -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 117016 | 0.66 | 0.748357 |
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Target: 5'- cGGGGCCGaguuucCACCCuCGgccucccccgaggGGAGGCCGg -3' miRNA: 3'- cCUCCGGU------GUGGGuGUa------------CCUCCGGUg -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 120133 | 0.66 | 0.723294 |
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Target: 5'- aGGAuccGUCGCAcgcCCCACAUGGcuGGCCGu -3' miRNA: 3'- -CCUc--CGGUGU---GGGUGUACCu-CCGGUg -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 77190 | 0.67 | 0.713509 |
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Target: 5'- gGGAGGCCcccuaguuACGCUU-CAUGGAGGUUg- -3' miRNA: 3'- -CCUCCGG--------UGUGGGuGUACCUCCGGug -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 113945 | 0.67 | 0.673781 |
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Target: 5'- ----aCCACAUCUACAUGcGGGCCACc -3' miRNA: 3'- ccuccGGUGUGGGUGUACcUCCGGUG- -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 140083 | 0.67 | 0.663746 |
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Target: 5'- aGGGGCCA-ACCCACAaaggccguuUGGAuuggcuGGCUGCg -3' miRNA: 3'- cCUCCGGUgUGGGUGU---------ACCU------CCGGUG- -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 114974 | 0.69 | 0.543555 |
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Target: 5'- gGGGGGCCGacaACCUGCucccGGGCCACc -3' miRNA: 3'- -CCUCCGGUg--UGGGUGuaccUCCGGUG- -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 144788 | 0.72 | 0.380447 |
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Target: 5'- gGGaAGGCacagcuccagCGCGCCCAUGUGGAGGaCCGa -3' miRNA: 3'- -CC-UCCG----------GUGUGGGUGUACCUCC-GGUg -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 114626 | 0.73 | 0.356575 |
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Target: 5'- aGGGGCCACACCgC-CggGGAGGCUuCa -3' miRNA: 3'- cCUCCGGUGUGG-GuGuaCCUCCGGuG- -5' |
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| 6256 | 3' | -59.4 | NC_001844.1 | + | 79040 | 0.73 | 0.333774 |
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Target: 5'- uGGaAGuGCCACAUCUGC--GGGGGCCACa -3' miRNA: 3'- -CC-UC-CGGUGUGGGUGuaCCUCCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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