Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6259 | 5' | -58.9 | NC_001844.1 | + | 137936 | 0.66 | 0.782227 |
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Target: 5'- -aGCgGGgGGCGCCGUCcacCAGCcCCAu -3' miRNA: 3'- caCG-CCgCCGUGGCAGac-GUUGuGGU- -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 115132 | 0.68 | 0.665247 |
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Target: 5'- cUGuCGGCGGCGaCGUUggGCAGCAgCAg -3' miRNA: 3'- cAC-GCCGCCGUgGCAGa-CGUUGUgGU- -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 56056 | 0.68 | 0.665247 |
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Target: 5'- -gGUGuGCGGCuCC-UCUgGCAACGCCAu -3' miRNA: 3'- caCGC-CGCCGuGGcAGA-CGUUGUGGU- -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 132148 | 0.68 | 0.644973 |
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Target: 5'- uUGCGcaCGGCACCGUUgaUGauuGCACCAa -3' miRNA: 3'- cACGCc-GCCGUGGCAG--ACgu-UGUGGU- -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 38939 | 0.69 | 0.584157 |
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Target: 5'- --cUGGCGGCGCUaauuUCUGCAGCACg- -3' miRNA: 3'- cacGCCGCCGUGGc---AGACGUUGUGgu -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 42451 | 0.69 | 0.554113 |
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Target: 5'- cUGCGGCGGC-CCGcCcGUucuCACCAg -3' miRNA: 3'- cACGCCGCCGuGGCaGaCGuu-GUGGU- -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 3615 | 0.7 | 0.544195 |
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Target: 5'- uGUGUGGC-GCACCGUUuagugcgGCAACACa- -3' miRNA: 3'- -CACGCCGcCGUGGCAGa------CGUUGUGgu -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 65526 | 0.71 | 0.476698 |
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Target: 5'- -aGCGGCGauacacacgcaaGCGCCGUCacgacUGgGACGCCAa -3' miRNA: 3'- caCGCCGC------------CGUGGCAG-----ACgUUGUGGU- -5' |
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| 6259 | 5' | -58.9 | NC_001844.1 | + | 12897 | 0.73 | 0.37995 |
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Target: 5'- uGUGCGGCGGCuACgCGacucaCUGCGGCACa- -3' miRNA: 3'- -CACGCCGCCG-UG-GCa----GACGUUGUGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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