Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6265 | 5' | -47 | NC_001844.1 | + | 105254 | 0.69 | 0.997 |
Target: 5'- cGGCUCCAuuAGGGCuGGUccgUUG-GCaCCa -3' miRNA: 3'- -CUGAGGUuuUCCUG-CCAa--AAUaCG-GG- -5' |
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6265 | 5' | -47 | NC_001844.1 | + | 90616 | 0.7 | 0.995075 |
Target: 5'- aGCUCUAAAAGccucGCGGUgaUGUGCUCc -3' miRNA: 3'- cUGAGGUUUUCc---UGCCAaaAUACGGG- -5' |
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6265 | 5' | -47 | NC_001844.1 | + | 79453 | 0.7 | 0.991061 |
Target: 5'- uGCUCCAGGAGGucGCGGUaaauaGCCg -3' miRNA: 3'- cUGAGGUUUUCC--UGCCAaaauaCGGg -5' |
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6265 | 5' | -47 | NC_001844.1 | + | 100654 | 0.71 | 0.986629 |
Target: 5'- aGAC-CCGccAGGGgGGUaaUAUGCCCg -3' miRNA: 3'- -CUGaGGUuuUCCUgCCAaaAUACGGG- -5' |
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6265 | 5' | -47 | NC_001844.1 | + | 133048 | 0.71 | 0.982845 |
Target: 5'- aACUCCAGcuauGGAUGGggu--UGCCCu -3' miRNA: 3'- cUGAGGUUuu--CCUGCCaaaauACGGG- -5' |
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6265 | 5' | -47 | NC_001844.1 | + | 64789 | 0.72 | 0.978306 |
Target: 5'- uGGCcaUCC-AAGGGGCGGUgggUGUGUCUg -3' miRNA: 3'- -CUG--AGGuUUUCCUGCCAaa-AUACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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