Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6266 | 3' | -46.4 | NC_001844.1 | + | 121032 | 0.66 | 0.999906 |
Target: 5'- cGGcgUGUUGGggGu-UAUGGUGGGAa -3' miRNA: 3'- -CUuaACGACCuuCucAUACCGCUUUg -5' |
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6266 | 3' | -46.4 | NC_001844.1 | + | 120868 | 0.66 | 0.999906 |
Target: 5'- ---gUGgUGGGAGGGU-UGGUGggGa -3' miRNA: 3'- cuuaACgACCUUCUCAuACCGCuuUg -5' |
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6266 | 3' | -46.4 | NC_001844.1 | + | 117617 | 0.68 | 0.999142 |
Target: 5'- ----aGCUGGGAGAGgccAUGGCcc-GCg -3' miRNA: 3'- cuuaaCGACCUUCUCa--UACCGcuuUG- -5' |
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6266 | 3' | -46.4 | NC_001844.1 | + | 43769 | 0.76 | 0.892593 |
Target: 5'- ----aGCUGGGAGGGgagguguUGGUGAAACu -3' miRNA: 3'- cuuaaCGACCUUCUCau-----ACCGCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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