Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6269 | 3' | -50.9 | NC_001844.1 | + | 19106 | 0.66 | 0.98885 |
Target: 5'- aGUuuGG-UGGCGCAG--GAAGAGGUc -3' miRNA: 3'- -CAggCCgAUCGCGUCauUUUUUCCGa -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 122627 | 0.66 | 0.98885 |
Target: 5'- -gCCGGCggcaGGCGCGGcGAcacAGGCg -3' miRNA: 3'- caGGCCGa---UCGCGUCaUUuuuUCCGa -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 117372 | 0.66 | 0.987299 |
Target: 5'- aUCCGcGCagccAGCGCGGccgAAGAGGGGUUc -3' miRNA: 3'- cAGGC-CGa---UCGCGUCa--UUUUUUCCGA- -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 144068 | 0.66 | 0.985587 |
Target: 5'- cGUCUGGCggAGCcCAGgc--GAAGGCa -3' miRNA: 3'- -CAGGCCGa-UCGcGUCauuuUUUCCGa -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 144520 | 0.66 | 0.983705 |
Target: 5'- -aUCGGUUGgccGCGCGGUGGcucuGGGCUg -3' miRNA: 3'- caGGCCGAU---CGCGUCAUUuuu-UCCGA- -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 79958 | 0.67 | 0.976935 |
Target: 5'- --gCGGgUGGCGCGGgucgcuGGGGCUg -3' miRNA: 3'- cagGCCgAUCGCGUCauuuu-UUCCGA- -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 13916 | 0.67 | 0.973717 |
Target: 5'- aUCCGGCUccGCGCAGUugccgcuuAGcGCUg -3' miRNA: 3'- cAGGCCGAu-CGCGUCAuuuuu---UC-CGA- -5' |
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6269 | 3' | -50.9 | NC_001844.1 | + | 58805 | 0.77 | 0.579892 |
Target: 5'- uGUCCGGCgGGCGCAGaacucGAGGCc -3' miRNA: 3'- -CAGGCCGaUCGCGUCauuuuUUCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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