Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6271 | 3' | -44.4 | NC_001844.1 | + | 59299 | 0.66 | 0.999994 |
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Target: 5'- --aGGCaCACGCGAGUgguguUUGCUGAu-- -3' miRNA: 3'- auaUUGaGUGCGCUCA-----AACGAUUugc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 21369 | 0.67 | 0.999992 |
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Target: 5'- cGUAACa-ACGgGAGUUUGCUcAAGCc -3' miRNA: 3'- aUAUUGagUGCgCUCAAACGA-UUUGc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 14931 | 0.68 | 0.99994 |
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Target: 5'- --cGGCaaggCGCGCGAGUUUuaUAGGCa -3' miRNA: 3'- auaUUGa---GUGCGCUCAAAcgAUUUGc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 54590 | 0.68 | 0.99994 |
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Target: 5'- --cGACcCGuCGCG-GUUUGCUGAGCu -3' miRNA: 3'- auaUUGaGU-GCGCuCAAACGAUUUGc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 100982 | 0.68 | 0.99992 |
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Target: 5'- gUGUAACUgccacgcccUACGCGAGcacGCUAAACu -3' miRNA: 3'- -AUAUUGA---------GUGCGCUCaaaCGAUUUGc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 137638 | 0.68 | 0.99992 |
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Target: 5'- --aGACUCGCGCGuccugcgcagGGUgUGUUGGACu -3' miRNA: 3'- auaUUGAGUGCGC----------UCAaACGAUUUGc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 51940 | 0.71 | 0.998553 |
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Target: 5'- --cAACUUggauguGCGCGAGUUUGUgauGACGu -3' miRNA: 3'- auaUUGAG------UGCGCUCAAACGau-UUGC- -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 30323 | 0.72 | 0.996761 |
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Target: 5'- aAUGGCUCGCGUGuugguuagcggugaGGUUgGCUGGACa -3' miRNA: 3'- aUAUUGAGUGCGC--------------UCAAaCGAUUUGc -5' |
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| 6271 | 3' | -44.4 | NC_001844.1 | + | 39947 | 0.76 | 0.967944 |
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Target: 5'- --aAACUCGCGUGAGUUauaGCUGcgGGCGg -3' miRNA: 3'- auaUUGAGUGCGCUCAAa--CGAU--UUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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