Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6273 | 3' | -50.1 | NC_001844.1 | + | 33824 | 0.66 | 0.994662 |
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Target: 5'- aUGg--CAGAAAGCGCUCCCgaACuCAa -3' miRNA: 3'- gACaagGUCUUUUGUGAGGGgaUG-GU- -5' |
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| 6273 | 3' | -50.1 | NC_001844.1 | + | 115049 | 0.66 | 0.993794 |
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Target: 5'- aCUGUUcCCGGGAAGC-CUCUCgUgguGCCGg -3' miRNA: 3'- -GACAA-GGUCUUUUGuGAGGGgA---UGGU- -5' |
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| 6273 | 3' | -50.1 | NC_001844.1 | + | 110731 | 0.67 | 0.990494 |
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Target: 5'- ----aUUGGAuuGCACUCCCCaACCAg -3' miRNA: 3'- gacaaGGUCUuuUGUGAGGGGaUGGU- -5' |
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| 6273 | 3' | -50.1 | NC_001844.1 | + | 94536 | 0.68 | 0.982102 |
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Target: 5'- uCUGaaCCAguaggcgacGAAAAgGCUCCCCgGCCAu -3' miRNA: 3'- -GACaaGGU---------CUUUUgUGAGGGGaUGGU- -5' |
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| 6273 | 3' | -50.1 | NC_001844.1 | + | 113730 | 0.68 | 0.981891 |
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Target: 5'- -cGggCCGGAGAGCGCacccgcgUUCCaCUGCCAc -3' miRNA: 3'- gaCaaGGUCUUUUGUG-------AGGG-GAUGGU- -5' |
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| 6273 | 3' | -50.1 | NC_001844.1 | + | 24962 | 0.71 | 0.925406 |
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Target: 5'- -aGUUUucgaaaUAGAAAACGCUUCaCCUACCAg -3' miRNA: 3'- gaCAAG------GUCUUUUGUGAGG-GGAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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