Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6279 | 3' | -49.9 | NC_001844.1 | + | 93625 | 0.66 | 0.993355 |
Target: 5'- uGACUCUCGUGUUGGgaAC-GCUGaCUa -3' miRNA: 3'- gUUGAGGGUAUAACCgaUGuUGGC-GA- -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 120985 | 0.66 | 0.993355 |
Target: 5'- gUAACUaCCUAUGcgguuUUGGUUGCGugUGCUu -3' miRNA: 3'- -GUUGA-GGGUAU-----AACCGAUGUugGCGA- -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 41523 | 0.66 | 0.992314 |
Target: 5'- aCGGCUUCCAgGUgggGGCggcuaccgGCAGCgGCUa -3' miRNA: 3'- -GUUGAGGGUaUAa--CCGa-------UGUUGgCGA- -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 87480 | 0.66 | 0.992314 |
Target: 5'- aAACUCCgGUAgcgcguauuaUUGGUUAUAacaaACCGCa -3' miRNA: 3'- gUUGAGGgUAU----------AACCGAUGU----UGGCGa -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 56279 | 0.67 | 0.98943 |
Target: 5'- cCAGCUCCCAUuauggagucaacggAgagGGCUGuguCAGCUGCc -3' miRNA: 3'- -GUUGAGGGUA--------------Uaa-CCGAU---GUUGGCGa -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 50993 | 0.67 | 0.988403 |
Target: 5'- gCGGuCUCCCcuug-GGCaGCAGCCGCc -3' miRNA: 3'- -GUU-GAGGGuauaaCCGaUGUUGGCGa -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 114464 | 0.68 | 0.983098 |
Target: 5'- uCGACcCCCAggcccUGGCUACcAUCGCa -3' miRNA: 3'- -GUUGaGGGUaua--ACCGAUGuUGGCGa -5' |
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6279 | 3' | -49.9 | NC_001844.1 | + | 106721 | 0.69 | 0.966619 |
Target: 5'- aCAACgcccaCCCAUAUcccgUGGCUACAuaagcaagcccuCCGCa -3' miRNA: 3'- -GUUGa----GGGUAUA----ACCGAUGUu-----------GGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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