Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6279 | 5' | -47.5 | NC_001844.1 | + | 142449 | 0.66 | 0.999601 |
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Target: 5'- cGGccgcCGUCUugCGCcGUGGCcCGAGGg -3' miRNA: 3'- -CCuu--GCAGAugGCGuCAUUGuGUUCU- -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 85443 | 0.67 | 0.999291 |
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Target: 5'- aGGGugGUCUACgccuacggcaacacaCGCcuGUAGCACAgcGGGg -3' miRNA: 3'- -CCUugCAGAUG---------------GCGu-CAUUGUGU--UCU- -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 137968 | 0.67 | 0.999228 |
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Target: 5'- uGGGGCGUgcgacggaucCUGCCGCAuGUcACAgCGGGGg -3' miRNA: 3'- -CCUUGCA----------GAUGGCGU-CAuUGU-GUUCU- -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 100470 | 0.67 | 0.99905 |
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Target: 5'- cGAACaaUUACUGCGuGUAACACAAGc -3' miRNA: 3'- cCUUGcaGAUGGCGU-CAUUGUGUUCu -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 137743 | 0.67 | 0.99905 |
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Target: 5'- gGGAGCGuUCUGcgcuCCGCcGUGGCGCccAGAc -3' miRNA: 3'- -CCUUGC-AGAU----GGCGuCAUUGUGu-UCU- -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 100270 | 0.68 | 0.997539 |
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Target: 5'- uGGAGCGcCUAUCGCGuuuGUGGgAuCAAGAg -3' miRNA: 3'- -CCUUGCaGAUGGCGU---CAUUgU-GUUCU- -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 136101 | 0.69 | 0.995207 |
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Target: 5'- cGGAGCG-CUACUG-GGUGGCGCugcuGGAc -3' miRNA: 3'- -CCUUGCaGAUGGCgUCAUUGUGu---UCU- -5' |
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| 6279 | 5' | -47.5 | NC_001844.1 | + | 140835 | 0.74 | 0.928398 |
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Target: 5'- cGGGAUGaCUACCGCcggGGUGGC-CAAGGg -3' miRNA: 3'- -CCUUGCaGAUGGCG---UCAUUGuGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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