Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 628 | 3' | -50.3 | AC_000017.1 | + | 13656 | 0.67 | 0.801001 |
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Target: 5'- uGCCUGCUcgcGCUGUUGcaacUCUagCaggGUCUGCg -3' miRNA: 3'- -CGGAUGA---UGACAAC----AGAa-Gg--CAGGCG- -5' |
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| 628 | 3' | -50.3 | AC_000017.1 | + | 15130 | 0.68 | 0.769602 |
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Target: 5'- -nCUGCcACUGUUGUUgccgCCGcCUGCg -3' miRNA: 3'- cgGAUGaUGACAACAGaa--GGCaGGCG- -5' |
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| 628 | 3' | -50.3 | AC_000017.1 | + | 15166 | 0.7 | 0.633347 |
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Target: 5'- uGCCgcgGCUGCcgcGUUGgaguucUCUUCCGcgCCGCu -3' miRNA: 3'- -CGGa--UGAUGa--CAAC------AGAAGGCa-GGCG- -5' |
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| 628 | 3' | -50.3 | AC_000017.1 | + | 2215 | 0.72 | 0.55229 |
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Target: 5'- -gCUGCUGCUGUUG-CUgcugcugcuccUCCGUCgGUa -3' miRNA: 3'- cgGAUGAUGACAACaGA-----------AGGCAGgCG- -5' |
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| 628 | 3' | -50.3 | AC_000017.1 | + | 26819 | 0.73 | 0.507311 |
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Target: 5'- gGCC-GCUGCUGUUG-CUgCCGcugCCGCc -3' miRNA: 3'- -CGGaUGAUGACAACaGAaGGCa--GGCG- -5' |
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| 628 | 3' | -50.3 | AC_000017.1 | + | 2108 | 1.14 | 0.000907 |
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Target: 5'- cGCCUACUACUGUUGUCUUCCGUCCGCc -3' miRNA: 3'- -CGGAUGAUGACAACAGAAGGCAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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