Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6287 | 5' | -58.6 | NC_001844.1 | + | 89416 | 0.66 | 0.758585 |
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Target: 5'- aUUCcUGaCUGGCCGGGCCagauauugucuuGCC-GUGa -3' miRNA: 3'- -AAGaACaGACCGGCCCGG------------UGGuCACc -5' |
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| 6287 | 5' | -58.6 | NC_001844.1 | + | 40794 | 0.67 | 0.709919 |
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Target: 5'- -cCUUGUCaagGGCgGcGGCCACgCuuuGUGGg -3' miRNA: 3'- aaGAACAGa--CCGgC-CCGGUG-Gu--CACC- -5' |
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| 6287 | 5' | -58.6 | NC_001844.1 | + | 50246 | 0.67 | 0.699953 |
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Target: 5'- aUCUcGUCUGGUUccugggGaGGCC-CCAGUGGc -3' miRNA: 3'- aAGAaCAGACCGG------C-CCGGuGGUCACC- -5' |
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| 6287 | 5' | -58.6 | NC_001844.1 | + | 130788 | 0.67 | 0.686915 |
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Target: 5'- aUUCUUGg--GGCCaauuguuuuauacuGGGCCACUuaaauGGUGGa -3' miRNA: 3'- -AAGAACagaCCGG--------------CCCGGUGG-----UCACC- -5' |
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| 6287 | 5' | -58.6 | NC_001844.1 | + | 82500 | 0.72 | 0.416999 |
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Target: 5'- aUCUUGcggCUGGgguaCGGGCUGCUGGUGGa -3' miRNA: 3'- aAGAACa--GACCg---GCCCGGUGGUCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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