Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6288 | 5' | -56.7 | NC_001844.1 | + | 110895 | 0.66 | 0.864482 |
Target: 5'- aGGCCagcaGCUGugCCaGCcgCCACUGgugGCCCGg -3' miRNA: 3'- -CUGGg---UGAUugGG-CG--GGUGAU---UGGGC- -5' |
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6288 | 5' | -56.7 | NC_001844.1 | + | 136257 | 0.66 | 0.866728 |
Target: 5'- aGACCCACgcugagcucagcACCCgccuccuuuuuugcGCCCACUGgugcuGCCUGg -3' miRNA: 3'- -CUGGGUGau----------UGGG--------------CGGGUGAU-----UGGGC- -5' |
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6288 | 5' | -56.7 | NC_001844.1 | + | 142456 | 0.66 | 0.879091 |
Target: 5'- gGACCCGCgg--CCGCCguCUugcgccguGGCCCGa -3' miRNA: 3'- -CUGGGUGauugGGCGGguGA--------UUGGGC- -5' |
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6288 | 5' | -56.7 | NC_001844.1 | + | 136340 | 0.66 | 0.879091 |
Target: 5'- -cCCCAU--GCCCGUuuuCCACUcggcgGACCCGc -3' miRNA: 3'- cuGGGUGauUGGGCG---GGUGA-----UUGGGC- -5' |
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6288 | 5' | -56.7 | NC_001844.1 | + | 114255 | 0.66 | 0.886069 |
Target: 5'- cGACCCGgcGGCUCGCgCCGggAGCCCc -3' miRNA: 3'- -CUGGGUgaUUGGGCG-GGUgaUUGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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