Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6289 | 3' | -61.9 | NC_001844.1 | + | 90792 | 0.66 | 0.679263 |
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Target: 5'- aCGGAggUGCUCCGCUGUauguuacGGGUGAGGa -3' miRNA: 3'- -GCCU--GCGAGGCGGCGcc-----UCCGCUUCa -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 141284 | 0.66 | 0.669523 |
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Target: 5'- gCGG-CGCUCgGCgaggGaUGGGGGCGAGGa -3' miRNA: 3'- -GCCuGCGAGgCGg---C-GCCUCCGCUUCa -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 138317 | 0.66 | 0.63036 |
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Target: 5'- uGuGGCGUaCCGUCGUGGGGGUGGucGUg -3' miRNA: 3'- gC-CUGCGaGGCGGCGCCUCCGCUu-CA- -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 140834 | 0.67 | 0.591212 |
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Target: 5'- gGGAUGaCUaCCGCCGgGGuGGCcAAGg -3' miRNA: 3'- gCCUGC-GA-GGCGGCgCCuCCGcUUCa -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 136318 | 0.68 | 0.542932 |
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Target: 5'- gCGGAccCGCUaCGCCGCGcGGuGGgGAGGUa -3' miRNA: 3'- -GCCU--GCGAgGCGGCGC-CU-CCgCUUCA- -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 114377 | 0.68 | 0.53342 |
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Target: 5'- gCGGAcCGCUCCGCCGCcG-GGCu---- -3' miRNA: 3'- -GCCU-GCGAGGCGGCGcCuCCGcuuca -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 140887 | 0.68 | 0.505264 |
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Target: 5'- cCGGAgacCGCUuugccuucCCGCCGCGGGGcuCGggGg -3' miRNA: 3'- -GCCU---GCGA--------GGCGGCGCCUCc-GCuuCa -5' |
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| 6289 | 3' | -61.9 | NC_001844.1 | + | 142573 | 1.07 | 0.001202 |
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Target: 5'- gCGGACGCUCCGCCGCGGAGGCGAAGUa -3' miRNA: 3'- -GCCUGCGAGGCGGCGCCUCCGCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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