Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
629 | 3' | -57.3 | AC_000017.1 | + | 5789 | 0.96 | 0.00401 |
Target: 5'- cUGUCCUCGAGCGGUguuCCGCGGUCCu -3' miRNA: 3'- -ACAGGAGCUCGCCAaacGGCGCCAGG- -5' |
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629 | 3' | -57.3 | AC_000017.1 | + | 17271 | 0.76 | 0.115154 |
Target: 5'- cG-CCUCG-GCGGUggcagaUGCCGCGGUgCa -3' miRNA: 3'- aCaGGAGCuCGCCAa-----ACGGCGCCAgG- -5' |
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629 | 3' | -57.3 | AC_000017.1 | + | 8536 | 0.75 | 0.14428 |
Target: 5'- gGUaCCgcgcggCGGGCGGUggGCCGCGGgggugUCCu -3' miRNA: 3'- aCA-GGa-----GCUCGCCAaaCGGCGCC-----AGG- -5' |
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629 | 3' | -57.3 | AC_000017.1 | + | 16338 | 0.68 | 0.366856 |
Target: 5'- gGUCCaggcgaCGAGCGGccgccgcagcaGCCGCGG-CCa -3' miRNA: 3'- aCAGGa-----GCUCGCCaaa--------CGGCGCCaGG- -5' |
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629 | 3' | -57.3 | AC_000017.1 | + | 9855 | 0.66 | 0.51714 |
Target: 5'- aUGUCCUUGGGUccGGccUGCUgaaugcgcagGCGGUCg -3' miRNA: 3'- -ACAGGAGCUCG--CCaaACGG----------CGCCAGg -5' |
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629 | 3' | -57.3 | AC_000017.1 | + | 30716 | 0.7 | 0.297019 |
Target: 5'- uUGUagCUaaGGCGGUggUUGCUGUGGUCCu -3' miRNA: 3'- -ACAg-GAgcUCGCCA--AACGGCGCCAGG- -5' |
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629 | 3' | -57.3 | AC_000017.1 | + | 15186 | 0.69 | 0.353044 |
Target: 5'- aUGUCCUCcacCGGcugcaUUGCCGCGGcugCCg -3' miRNA: 3'- -ACAGGAGcucGCCa----AACGGCGCCa--GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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