Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6290 | 5' | -56.1 | NC_001844.1 | + | 38491 | 0.66 | 0.905692 |
Target: 5'- aCGCgagcuagauaUCUgCGUcGGUGggGGACCC-UGUa -3' miRNA: 3'- -GCG----------AGGgGCA-CUACuuCCUGGGuACA- -5' |
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6290 | 5' | -56.1 | NC_001844.1 | + | 141815 | 0.66 | 0.899369 |
Target: 5'- -cCUCCCCgGUGGUGggGcguccGACCCGg-- -3' miRNA: 3'- gcGAGGGG-CACUACuuC-----CUGGGUaca -5' |
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6290 | 5' | -56.1 | NC_001844.1 | + | 141465 | 0.7 | 0.691616 |
Target: 5'- aGCUCCCgG-GgcGAGGGGCCCGc-- -3' miRNA: 3'- gCGAGGGgCaCuaCUUCCUGGGUaca -5' |
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6290 | 5' | -56.1 | NC_001844.1 | + | 142184 | 0.76 | 0.358256 |
Target: 5'- gGCUgCCgCGUGcgGAAGGGCCCAUa- -3' miRNA: 3'- gCGAgGG-GCACuaCUUCCUGGGUAca -5' |
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6290 | 5' | -56.1 | NC_001844.1 | + | 142108 | 1.07 | 0.00347 |
Target: 5'- aCGCUCCCCGUGAUGAAGGACCCAUGUc -3' miRNA: 3'- -GCGAGGGGCACUACUUCCUGGGUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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