Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6300 | 5' | -54.8 | NC_001844.1 | + | 125984 | 0.66 | 0.910098 |
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Target: 5'- gCAGCU--UAUCACAGCuuuacagguggUGGCACUACAg -3' miRNA: 3'- -GUCGAcuGUGGUGUCG-----------ACUGUGGUGU- -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 137546 | 0.66 | 0.903798 |
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Target: 5'- gCAGCUGAUGCCGgAGCaGAgccuucCGCUGCGg -3' miRNA: 3'- -GUCGACUGUGGUgUCGaCU------GUGGUGU- -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 89342 | 0.66 | 0.896586 |
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Target: 5'- -uGCUG-CACCACAucuaaccGCUGACGCUcuaauGCGg -3' miRNA: 3'- guCGACuGUGGUGU-------CGACUGUGG-----UGU- -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 114390 | 0.67 | 0.883441 |
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Target: 5'- gAGCUGGCugC-CAGCgGACcgcuccGCCGCc -3' miRNA: 3'- gUCGACUGugGuGUCGaCUG------UGGUGu -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 102146 | 0.68 | 0.828032 |
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Target: 5'- cCAGCggcGGCACCAUAGCUGGagaaACAg -3' miRNA: 3'- -GUCGa--CUGUGGUGUCGACUguggUGU- -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 3906 | 0.69 | 0.763524 |
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Target: 5'- aAGCUGGgGCUACuGGUgGACAUCACAa -3' miRNA: 3'- gUCGACUgUGGUG-UCGaCUGUGGUGU- -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 68436 | 0.69 | 0.763524 |
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Target: 5'- uGGCcGACGCCcugGCAGCccuggaaGACGCCGCGc -3' miRNA: 3'- gUCGaCUGUGG---UGUCGa------CUGUGGUGU- -5' |
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| 6300 | 5' | -54.8 | NC_001844.1 | + | 39110 | 0.72 | 0.609421 |
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Target: 5'- aCGGUUGACACCACuuGCUuuggGGCAUCAUg -3' miRNA: 3'- -GUCGACUGUGGUGu-CGA----CUGUGGUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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