Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6301 | 3' | -57.7 | NC_001844.1 | + | 92969 | 0.66 | 0.853267 |
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Target: 5'- --gGGCGCGUaaaaacGGUGUCgUCAAagGCGCg -3' miRNA: 3'- gaaCCGCGCAa-----CCACGG-GGUUg-CGCG- -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 1207 | 0.66 | 0.845405 |
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Target: 5'- -gUGGCuGUGUUguggaGGUGCacacCCCAGCGaUGCa -3' miRNA: 3'- gaACCG-CGCAA-----CCACG----GGGUUGC-GCG- -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 31689 | 0.66 | 0.829116 |
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Target: 5'- -gUGGCGUG-UGGUGUUCaagAGgGCGCc -3' miRNA: 3'- gaACCGCGCaACCACGGGg--UUgCGCG- -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 144657 | 0.66 | 0.829116 |
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Target: 5'- --cGGCGUc--GG-GCUCCAGCaGCGCg -3' miRNA: 3'- gaaCCGCGcaaCCaCGGGGUUG-CGCG- -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 4463 | 0.68 | 0.735506 |
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Target: 5'- cCUUGGCGCGcauagcucgggccgUUGGgagugGCgCCGAgGCGg -3' miRNA: 3'- -GAACCGCGC--------------AACCa----CGgGGUUgCGCg -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 141394 | 0.68 | 0.709024 |
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Target: 5'- --cGGgGCuUUGGUGCCCCuucucugaGCGUc -3' miRNA: 3'- gaaCCgCGcAACCACGGGGuug-----CGCG- -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 65631 | 0.7 | 0.638615 |
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Target: 5'- --cGGCGCuuGUUGGaGCCCUAGC-UGCa -3' miRNA: 3'- gaaCCGCG--CAACCaCGGGGUUGcGCG- -5' |
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| 6301 | 3' | -57.7 | NC_001844.1 | + | 129544 | 0.77 | 0.267262 |
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Target: 5'- ---cGCGCGUUGGgGCaCCAACGCGCc -3' miRNA: 3'- gaacCGCGCAACCaCGgGGUUGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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