Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6305 | 3' | -57 | NC_001844.1 | + | 34262 | 0.66 | 0.869752 |
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Target: 5'- uUC-CACGCCCuuGUCUGCCaaaCCGa-- -3' miRNA: 3'- -AGaGUGUGGGggCAGAUGGc--GGUacc -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 54776 | 0.67 | 0.846988 |
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Target: 5'- --gCGCACCCCaaaaGUgUACUGUgAUGGc -3' miRNA: 3'- agaGUGUGGGGg---CAgAUGGCGgUACC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 115289 | 0.67 | 0.845408 |
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Target: 5'- -gUCGCACCCCUGgugcgCUACaacgcggagggaGCCcgGGc -3' miRNA: 3'- agAGUGUGGGGGCa----GAUGg-----------CGGuaCC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 127098 | 0.67 | 0.822519 |
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Target: 5'- --aCACACCCCCGguguggaUGCUGUCAucgugcUGGa -3' miRNA: 3'- agaGUGUGGGGGCag-----AUGGCGGU------ACC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 104029 | 0.67 | 0.821676 |
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Target: 5'- aUUUCACACCCCUuauagcuuauauuGUCUACaaacgCGUCAaaUGGg -3' miRNA: 3'- -AGAGUGUGGGGG-------------CAGAUG-----GCGGU--ACC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 142157 | 0.67 | 0.814016 |
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Target: 5'- ---aGCAUUCCCG---GCCGCCAUGGc -3' miRNA: 3'- agagUGUGGGGGCagaUGGCGGUACC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 115366 | 0.68 | 0.796536 |
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Target: 5'- aCUCugACCCUa-UCcACCGCC-UGGg -3' miRNA: 3'- aGAGugUGGGGgcAGaUGGCGGuACC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 48353 | 0.73 | 0.493442 |
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Target: 5'- uUUUCACGCCCCCGauaaUCguaCGCCAgGGg -3' miRNA: 3'- -AGAGUGUGGGGGC----AGaugGCGGUaCC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 142452 | 0.77 | 0.31766 |
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Target: 5'- --cCGCGgCCgCCGUCUugCGCCGUGGc -3' miRNA: 3'- agaGUGUgGG-GGCAGAugGCGGUACC- -5' |
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| 6305 | 3' | -57 | NC_001844.1 | + | 121519 | 1.11 | 0.001715 |
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Target: 5'- uUCUCACACCCCCGUCUACCGCCAUGGg -3' miRNA: 3'- -AGAGUGUGGGGGCAGAUGGCGGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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