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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6307 | 3' | -54.3 | NC_001844.1 | + | 140839 | 0.68 | 0.885865 |
Target: 5'- -gCGGCGGGAuGACUAccGCCGG-GGUGg -3' miRNA: 3'- uaGCUGCCUU-CUGGUu-CGGCUaCCAC- -5' |
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6307 | 3' | -54.3 | NC_001844.1 | + | 116924 | 1.06 | 0.006293 |
Target: 5'- cAUCGACGGAAGACCAAGCCGAUGGUGc -3' miRNA: 3'- -UAGCUGCCUUCUGGUUCGGCUACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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