Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6309 | 3' | -55.6 | NC_001844.1 | + | 56182 | 0.66 | 0.900479 |
Target: 5'- cGCUAccGUCCCccUGGCGCUgagUGCGc -3' miRNA: 3'- -UGGUuuCGGGGcaGCCGCGAaa-ACGC- -5' |
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6309 | 3' | -55.6 | NC_001844.1 | + | 144464 | 0.66 | 0.893911 |
Target: 5'- aACCcGAGCCCC-UCGGC-CUgg-GCu -3' miRNA: 3'- -UGGuUUCGGGGcAGCCGcGAaaaCGc -5' |
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6309 | 3' | -55.6 | NC_001844.1 | + | 117235 | 0.67 | 0.872825 |
Target: 5'- gGCCcccGAGCCCCG-CGGCGggaagGCa -3' miRNA: 3'- -UGGu--UUCGGGGCaGCCGCgaaaaCGc -5' |
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6309 | 3' | -55.6 | NC_001844.1 | + | 144531 | 0.68 | 0.816238 |
Target: 5'- cCCAAGGCCCCaucgGUUGGcCGCgcg-GUGg -3' miRNA: 3'- uGGUUUCGGGG----CAGCC-GCGaaaaCGC- -5' |
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6309 | 3' | -55.6 | NC_001844.1 | + | 120907 | 0.75 | 0.460081 |
Target: 5'- -aCAGGGCgCCCG-CGGCGCUUUUGa- -3' miRNA: 3'- ugGUUUCG-GGGCaGCCGCGAAAACgc -5' |
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6309 | 3' | -55.6 | NC_001844.1 | + | 116700 | 1.09 | 0.002723 |
Target: 5'- cACCAAAGCCCCGUCGGCGCUUUUGCGg -3' miRNA: 3'- -UGGUUUCGGGGCAGCCGCGAAAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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