Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6310 | 3' | -58.7 | NC_001844.1 | + | 141459 | 0.66 | 0.792911 |
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Target: 5'- -cGGgGC--GAGggGCCCG-CCCGGGu -3' miRNA: 3'- uuCUgCGacCUCuuCGGGCuGGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 142865 | 0.67 | 0.733756 |
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Target: 5'- -cGGC-CUGGGugaugguccaggccGAGGCCCGgGCCCGGGc -3' miRNA: 3'- uuCUGcGACCU--------------CUUCGGGC-UGGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 141753 | 0.67 | 0.707219 |
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Target: 5'- --cGCGCUGGcAGGuuccgAGCCCuggGGCCUGAGa -3' miRNA: 3'- uucUGCGACC-UCU-----UCGGG---CUGGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 113319 | 0.67 | 0.707219 |
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Target: 5'- uGGGcGCGCUGGAGcuguGCCUu-CCCGAGu -3' miRNA: 3'- -UUC-UGCGACCUCuu--CGGGcuGGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 141809 | 0.67 | 0.687258 |
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Target: 5'- cGGugG-UGGGGc-GUCCGACCCGGGc -3' miRNA: 3'- uUCugCgACCUCuuCGGGCUGGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 115268 | 0.7 | 0.526444 |
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Target: 5'- --aACGC-GGAgGGAGCCCGgGCCCGGGc -3' miRNA: 3'- uucUGCGaCCU-CUUCGGGC-UGGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 121982 | 0.71 | 0.487969 |
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Target: 5'- cGAGuCgGCUGGGGggGucucCCCGAuCCCGAGu -3' miRNA: 3'- -UUCuG-CGACCUCuuC----GGGCU-GGGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 113447 | 0.76 | 0.2659 |
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Target: 5'- --cGCGCUGcuGGAGCCCGACgCCGAGg -3' miRNA: 3'- uucUGCGACcuCUUCGGGCUG-GGCUC- -5' |
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| 6310 | 3' | -58.7 | NC_001844.1 | + | 116573 | 1.06 | 0.00215 |
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Target: 5'- cAAGACGCUGGAGAAGCCCGACCCGAGa -3' miRNA: 3'- -UUCUGCGACCUCUUCGGGCUGGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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