Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6314 | 3' | -56.2 | NC_001844.1 | + | 143863 | 0.69 | 0.76508 |
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Target: 5'- cGG-ACCAGAgggGCUCCCGgcgcgAGCC-GCCGg -3' miRNA: 3'- aCCaUGGUCU---UGGGGGU-----UCGGaUGGC- -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 116056 | 0.69 | 0.736076 |
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Target: 5'- gGGgccgACCAGAACCuggCCCAGuUCUGCCa -3' miRNA: 3'- aCCa---UGGUCUUGG---GGGUUcGGAUGGc -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 80975 | 0.7 | 0.706253 |
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Target: 5'- gGGUugCAGggUCgCCUAaaauaauuaaGGUCUGCCGa -3' miRNA: 3'- aCCAugGUCuuGG-GGGU----------UCGGAUGGC- -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 74782 | 0.7 | 0.706253 |
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Target: 5'- cGuUACCAG-ACCCCCAccugguGGCCgaUGCCGu -3' miRNA: 3'- aCcAUGGUCuUGGGGGU------UCGG--AUGGC- -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 143251 | 0.71 | 0.634872 |
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Target: 5'- gGGUGCCGGuccGCCCCCuc-CCUGCg- -3' miRNA: 3'- aCCAUGGUCu--UGGGGGuucGGAUGgc -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 113513 | 0.72 | 0.543238 |
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Target: 5'- cGGaGCCGGGGCCgUgGAGCCcGCCGa -3' miRNA: 3'- aCCaUGGUCUUGGgGgUUCGGaUGGC- -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 114666 | 0.73 | 0.494128 |
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Target: 5'- gGGUACUAGGcCCCUCGAGCUuuggggcccaUGCCGc -3' miRNA: 3'- aCCAUGGUCUuGGGGGUUCGG----------AUGGC- -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 142465 | 0.79 | 0.25535 |
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Target: 5'- cUGGUACCAGGACCCgCG-GCC-GCCGu -3' miRNA: 3'- -ACCAUGGUCUUGGGgGUuCGGaUGGC- -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 35440 | 0.81 | 0.194954 |
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Target: 5'- gGGUGCCucGGAGCCCCCcagcAGCUUACCa -3' miRNA: 3'- aCCAUGG--UCUUGGGGGu---UCGGAUGGc -5' |
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| 6314 | 3' | -56.2 | NC_001844.1 | + | 115627 | 1.08 | 0.003019 |
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Target: 5'- cUGGUACCAGAACCCCCAAGCCUACCGc -3' miRNA: 3'- -ACCAUGGUCUUGGGGGUUCGGAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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