miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
6315 3' -60.1 NC_001844.1 + 107808 0.66 0.700988
Target:  5'- -gGC-CCucCUCCGCUGGGCUguuuugCGGGGc -3'
miRNA:   3'- caCGaGGuuGGGGCGACCCGA------GCUCU- -5'
6315 3' -60.1 NC_001844.1 + 116369 0.66 0.69109
Target:  5'- cUGCUCCucucaGGCCCCa--GGGCUCG-GAa -3'
miRNA:   3'- cACGAGG-----UUGGGGcgaCCCGAGCuCU- -5'
6315 3' -60.1 NC_001844.1 + 141128 0.68 0.561008
Target:  5'- aUGCUCCGGCCCC-UUGGcGCcggccuccccUCGGGGg -3'
miRNA:   3'- cACGAGGUUGGGGcGACC-CG----------AGCUCU- -5'
6315 3' -60.1 NC_001844.1 + 140896 0.69 0.53744
Target:  5'- cUGCUCUggccggaGACCgcuuugccuucccgCCGCgGGGCUCGGGGg -3'
miRNA:   3'- cACGAGG-------UUGG--------------GGCGaCCCGAGCUCU- -5'
6315 3' -60.1 NC_001844.1 + 117169 0.76 0.224331
Target:  5'- -cGCUCaUAACCCCGCUccgGGGCUcgcCGAGAu -3'
miRNA:   3'- caCGAG-GUUGGGGCGA---CCCGA---GCUCU- -5'
6315 3' -60.1 NC_001844.1 + 115453 1.07 0.001332
Target:  5'- uGUGCUCCAACCCCGCUGGGCUCGAGAc -3'
miRNA:   3'- -CACGAGGUUGGGGCGACCCGAGCUCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.