Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6317 | 3' | -56.9 | NC_001844.1 | + | 141459 | 0.66 | 0.88028 |
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Target: 5'- -cGGGGCGaGGGGcccGCCCGGGUuacuCGGGGu -3' miRNA: 3'- guUCCUGUaCUCU---CGGGUCCG----GCUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 117047 | 0.66 | 0.873124 |
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Target: 5'- gGAGcGACAccGAGAccuGCCCGGGgucucCCGGGGc -3' miRNA: 3'- gUUC-CUGUa-CUCU---CGGGUCC-----GGCUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 115402 | 0.67 | 0.834247 |
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Target: 5'- uCAGGGACcUGGucGAGCgugCCAGGCuacuCGGGGa -3' miRNA: 3'- -GUUCCUGuACU--CUCG---GGUCCG----GCUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 113454 | 0.67 | 0.799824 |
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Target: 5'- gCAGGGucGCGcUGcuGGAGCCCGacGCCGAGGc -3' miRNA: 3'- -GUUCC--UGU-AC--UCUCGGGUc-CGGCUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 116511 | 0.68 | 0.753477 |
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Target: 5'- cCAAGGACc--AGcGGCCCAagucugcguuGGCCGGGGc -3' miRNA: 3'- -GUUCCUGuacUC-UCGGGU----------CCGGCUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 142761 | 0.69 | 0.684387 |
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Target: 5'- gCGAGGcGCAgcucgucGGGGCCCAGGCgGuGGa -3' miRNA: 3'- -GUUCC-UGUac-----UCUCGGGUCCGgCuCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 115264 | 0.7 | 0.633563 |
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Target: 5'- --cGGA---GGGAGCCCGGGCCcGGGc -3' miRNA: 3'- guuCCUguaCUCUCGGGUCCGGcUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 142860 | 0.72 | 0.532708 |
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Target: 5'- -uGGGugAU---GGUCCAGGCCGAGGc -3' miRNA: 3'- guUCCugUAcucUCGGGUCCGGCUCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 113522 | 0.73 | 0.484336 |
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Target: 5'- --cGGACGgcgcGGAGCCgGGGCCGuGGa -3' miRNA: 3'- guuCCUGUac--UCUCGGgUCCGGCuCC- -5' |
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| 6317 | 3' | -56.9 | NC_001844.1 | + | 113655 | 1.11 | 0.001752 |
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Target: 5'- gCAAGGACAUGAGAGCCCAGGCCGAGGg -3' miRNA: 3'- -GUUCCUGUACUCUCGGGUCCGGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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