Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6318 | 5' | -60.6 | NC_001844.1 | + | 144472 | 0.66 | 0.720405 |
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Target: 5'- gGUCGuggaacCCGAGCCCCUCGGccUGGGc-- -3' miRNA: 3'- -CGGC------GGCUCGGGGAGCCcaACUCacc -5' |
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| 6318 | 5' | -60.6 | NC_001844.1 | + | 143474 | 0.66 | 0.701226 |
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Target: 5'- cCCGgCGguguGGCCCCUCGGGcgGcaUGGg -3' miRNA: 3'- cGGCgGC----UCGGGGAGCCCaaCucACC- -5' |
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| 6318 | 5' | -60.6 | NC_001844.1 | + | 143848 | 0.69 | 0.574048 |
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Target: 5'- cCCgGCgCGAGCCgC-CGGGUcGGGUGGu -3' miRNA: 3'- cGG-CG-GCUCGGgGaGCCCAaCUCACC- -5' |
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| 6318 | 5' | -60.6 | NC_001844.1 | + | 140943 | 0.71 | 0.435328 |
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Target: 5'- cUCGgCGAGCCCCggagCGGGguuaUGAGcGGg -3' miRNA: 3'- cGGCgGCUCGGGGa---GCCCa---ACUCaCC- -5' |
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| 6318 | 5' | -60.6 | NC_001844.1 | + | 141114 | 0.72 | 0.401635 |
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Target: 5'- uGgCGCCGGccuCCCCUCGGGggaggccgaGGGUGGa -3' miRNA: 3'- -CgGCGGCUc--GGGGAGCCCaa-------CUCACC- -5' |
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| 6318 | 5' | -60.6 | NC_001844.1 | + | 113386 | 1.12 | 0.000715 |
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Target: 5'- cGCCGCCGAGCCCCUCGGGUUGAGUGGg -3' miRNA: 3'- -CGGCGGCUCGGGGAGCCCAACUCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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