Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6320 | 3' | -48.1 | NC_001846.1 | + | 26113 | 0.66 | 0.91601 |
Target: 5'- -uGAUAgUGUCCAAUCCGuuGAUGGa -3' miRNA: 3'- auCUGUaGCAGGUUAGGUucCUAUCg -5' |
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6320 | 3' | -48.1 | NC_001846.1 | + | 5107 | 0.68 | 0.841146 |
Target: 5'- aAGACAUUagGUUCAGUCUuuuGuGAUGGCa -3' miRNA: 3'- aUCUGUAG--CAGGUUAGGuu-C-CUAUCG- -5' |
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6320 | 3' | -48.1 | NC_001846.1 | + | 2176 | 0.68 | 0.821309 |
Target: 5'- gUGGACAUUGcugCUAGUgCCGcuGGAUGGCu -3' miRNA: 3'- -AUCUGUAGCa--GGUUA-GGUu-CCUAUCG- -5' |
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6320 | 3' | -48.1 | NC_001846.1 | + | 1837 | 1.12 | 0.001722 |
Target: 5'- aUAGACAUCGUCCAAUCCAAGGAUAGCu -3' miRNA: 3'- -AUCUGUAGCAGGUUAGGUUCCUAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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