miRNA: 3'- -CGUa---CCaAACGUuaCGUCU-GUgauGG- -5'
miRNA: 3'- cGUACcaaaCGU------UACGUCUGUGAUGg -5'
miRNA: 3'- -CGUACCAaa--CGUuaCGUCUgugaUGG- -5'
miRNA: 3'- cguaCCAaACGUUA------CGUCugUGAUGG- -5'
miRNA: 3'- -CGUACCAAACGUUACGUCUGUGAUGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6329 | 5' | -46.6 | NC_001846.1 | + | 22079 | 0.66 | 0.95259 |
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Target: 5'- cGCAgaagGGgUUGCAcgGCAGAuCAau-CCa -3' miRNA: 3'- -CGUa---CCaAACGUuaCGUCU-GUgauGG- -5' |
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| 6329 | 5' | -46.6 | NC_001846.1 | + | 18817 | 0.68 | 0.895477 |
|
Target: 5'- aCAUGuc--GCAcgcuugGUGCAGACACUACa -3' miRNA: 3'- cGUACcaaaCGU------UACGUCUGUGAUGg -5' |
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| 6329 | 5' | -46.6 | NC_001846.1 | + | 877 | 0.69 | 0.870405 |
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Target: 5'- gGCAUGGUaacaGCGcgGCGGAagugGCCg -3' miRNA: 3'- -CGUACCAaa--CGUuaCGUCUgugaUGG- -5' |
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| 6329 | 5' | -46.6 | NC_001846.1 | + | 21065 | 0.74 | 0.576221 |
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Target: 5'- ---gGGUcUGCAGUucuuagGCAGugGCUACCa -3' miRNA: 3'- cguaCCAaACGUUA------CGUCugUGAUGG- -5' |
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| 6329 | 5' | -46.6 | NC_001846.1 | + | 12538 | 1.16 | 0.001244 |
|
Target: 5'- aGCAUGGUUUGCAAUGCAGACACUACCu -3' miRNA: 3'- -CGUACCAAACGUUACGUCUGUGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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