Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6344 | 3' | -54.8 | NC_001847.1 | + | 12322 | 0.71 | 0.665606 |
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Target: 5'- cGGUGGgCGCGCccGCcgGCUcgcUGGUGAg- -3' miRNA: 3'- -CUACCgGCGCG--UGuaCGA---ACCACUag -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 55049 | 0.72 | 0.595939 |
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Target: 5'- aGGUGGCCGCGUucgucgcgcgguucaGCcgGC-UGGUGggCa -3' miRNA: 3'- -CUACCGGCGCG---------------UGuaCGaACCACuaG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 70659 | 0.73 | 0.550024 |
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Target: 5'- cGUGGCCGCGCAUcUGCUggGGcUGGa- -3' miRNA: 3'- cUACCGGCGCGUGuACGAa-CC-ACUag -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 134519 | 0.73 | 0.5295 |
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Target: 5'- --cGGCCGCgGCGCAUGU--GGUGGUa -3' miRNA: 3'- cuaCCGGCG-CGUGUACGaaCCACUAg -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 126782 | 0.73 | 0.509264 |
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Target: 5'- ---uGCCGCGCccuACGUGUUUGGUcGAUCg -3' miRNA: 3'- cuacCGGCGCG---UGUACGAACCA-CUAG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 59505 | 0.74 | 0.469827 |
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Target: 5'- gGGUGcGCCGaggcCGCACcgGCUUGGUGcgCu -3' miRNA: 3'- -CUAC-CGGC----GCGUGuaCGAACCACuaG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 6154 | 0.8 | 0.229232 |
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Target: 5'- cGGUGGCggugacggCGCGCAgGUGCUcGGUGAUCu -3' miRNA: 3'- -CUACCG--------GCGCGUgUACGAaCCACUAG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 79251 | 0.81 | 0.195273 |
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Target: 5'- aAUGGCCGCGUACGUGCUgcccgcccaggucgUGGUcacGAUCa -3' miRNA: 3'- cUACCGGCGCGUGUACGA--------------ACCA---CUAG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 135012 | 1.09 | 0.002473 |
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Target: 5'- aGAUGGCCGCGCACAUGCUUGGUGAUCu -3' miRNA: 3'- -CUACCGGCGCGUGUACGAACCACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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