Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 636 | 3' | -55.4 | AC_000017.1 | + | 9747 | 0.66 | 0.593628 |
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Target: 5'- -gUgGCGGGCGGcagcgGGCGGCG-GUCg- -3' miRNA: 3'- gaAgUGCCUGUCa----CCGUCGCaCAGgg -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 13137 | 0.66 | 0.58689 |
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Target: 5'- -cUCuCGGuACAGUGuCAGCaagugaccuagguauGUGUCCCg -3' miRNA: 3'- gaAGuGCC-UGUCACcGUCG---------------CACAGGG- -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 3873 | 0.67 | 0.538034 |
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Target: 5'- --cCGCGGGCGGUGGCugcAGCGg--CUg -3' miRNA: 3'- gaaGUGCCUGUCACCG---UCGCacaGGg -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 17598 | 0.67 | 0.526025 |
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Target: 5'- --gCACGGGCuggcgacggcgacGGcGGCGGCGgGUUCCa -3' miRNA: 3'- gaaGUGCCUG-------------UCaCCGUCGCaCAGGG- -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 27993 | 0.67 | 0.516276 |
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Target: 5'- --cCGCGGGCAaagcacuuGUGGCGGCGgcagugGUCa- -3' miRNA: 3'- gaaGUGCCUGU--------CACCGUCGCa-----CAGgg -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 9132 | 0.68 | 0.494881 |
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Target: 5'- -----aGGGUGGUGGCGGUGUGUUCUg -3' miRNA: 3'- gaagugCCUGUCACCGUCGCACAGGG- -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 8546 | 0.7 | 0.367505 |
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Target: 5'- ---gGCGGGCGGUgGGCcgcgGGgGUGUCCUu -3' miRNA: 3'- gaagUGCCUGUCA-CCG----UCgCACAGGG- -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 14210 | 0.72 | 0.264534 |
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Target: 5'- --gCGCGGcgcCAGUGGCGGCGgcgcuggGUUCCc -3' miRNA: 3'- gaaGUGCCu--GUCACCGUCGCa------CAGGG- -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 25384 | 0.73 | 0.250944 |
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Target: 5'- --cCAUGGGCGuGUGGCAGCaGUG-CCUg -3' miRNA: 3'- gaaGUGCCUGU-CACCGUCG-CACaGGG- -5' |
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| 636 | 3' | -55.4 | AC_000017.1 | + | 13035 | 1.13 | 0.000315 |
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Target: 5'- cCUUCACGGACAGUGGCAGCGUGUCCCg -3' miRNA: 3'- -GAAGUGCCUGUCACCGUCGCACAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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