Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6365 | 3' | -48.9 | NC_001847.1 | + | 130184 | 0.7 | 0.956519 |
Target: 5'- cGCGGGCGCCCggccUGAAGAgacggGcgGCgCGg -3' miRNA: 3'- uUGCCUGCGGGa---AUUUUUa----CuaCG-GC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 52929 | 0.72 | 0.915526 |
Target: 5'- --gGGGCGCCCgcGAAGGccGUGCCGg -3' miRNA: 3'- uugCCUGCGGGaaUUUUUacUACGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 116865 | 0.91 | 0.143863 |
Target: 5'- aAACGGACGCCCUUAAAAAUGuaGCCGc -3' miRNA: 3'- -UUGCCUGCGGGAAUUUUUACuaCGGC- -5' |
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6365 | 3' | -48.9 | NC_001847.1 | + | 123638 | 0.66 | 0.998002 |
Target: 5'- gGGCGG-CGCCCUUGGcuGAA-GgcGCCu -3' miRNA: 3'- -UUGCCuGCGGGAAUU--UUUaCuaCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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