Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 637 | 5' | -59.1 | AC_000017.1 | + | 13803 | 0.73 | 0.110999 |
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Target: 5'- uAGCAGGCGcGGGCgGGUGgUGCGAGu- -3' miRNA: 3'- -UCGUCCGUcUCCG-CCGCgACGCUUuc -5' |
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| 637 | 5' | -59.1 | AC_000017.1 | + | 9748 | 0.73 | 0.106917 |
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Target: 5'- uGGCGGGCGGcagcGGGCGGCGgUcgggguuguuucugGCGGAGGu -3' miRNA: 3'- -UCGUCCGUC----UCCGCCGCgA--------------CGCUUUC- -5' |
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| 637 | 5' | -59.1 | AC_000017.1 | + | 17279 | 0.74 | 0.104778 |
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Target: 5'- cGguGGCAGAugccgcggugcaGGCGGcCGCUGCGGc-- -3' miRNA: 3'- uCguCCGUCU------------CCGCC-GCGACGCUuuc -5' |
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| 637 | 5' | -59.1 | AC_000017.1 | + | 15055 | 0.74 | 0.098886 |
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Target: 5'- --aGGGCGGGGGUGGCGCagGCGgcGGc -3' miRNA: 3'- ucgUCCGUCUCCGCCGCGa-CGCuuUC- -5' |
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| 637 | 5' | -59.1 | AC_000017.1 | + | 8673 | 0.74 | 0.090632 |
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Target: 5'- cGCGGGCAGGaGCuGGUGCUGCGcacGGAGg -3' miRNA: 3'- uCGUCCGUCUcCG-CCGCGACGC---UUUC- -5' |
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| 637 | 5' | -59.1 | AC_000017.1 | + | 13613 | 1.09 | 0.000187 |
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Target: 5'- gAGCAGGCAGAGGCGGCGCUGCGAAAGg -3' miRNA: 3'- -UCGUCCGUCUCCGCCGCGACGCUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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