Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6370 | 3' | -54.6 | NC_001847.1 | + | 102412 | 0.77 | 0.352183 |
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Target: 5'- uAGCCGCcGGCcGGAGGCCGAGa--- -3' miRNA: 3'- uUUGGCGaCCGaCCUCUGGCUCaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 102669 | 0.66 | 0.899887 |
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Target: 5'- cGGCCGCgagGGCgGGGGGgCGGGg--- -3' miRNA: 3'- uUUGGCGa--CCGaCCUCUgGCUCaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 108453 | 0.68 | 0.839342 |
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Target: 5'- cGAGCUGC-GGCUGGAGcCCGccGGcgUCa -3' miRNA: 3'- -UUUGGCGaCCGACCUCuGGC--UCaaAG- -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 111783 | 1.06 | 0.004226 |
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Target: 5'- cAAACCGCUGGCUGGAGACCGAGUUUCc -3' miRNA: 3'- -UUUGGCGACCGACCUCUGGCUCAAAG- -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 130097 | 0.66 | 0.918636 |
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Target: 5'- cGACCGCgacgcgGGCUGG-GAC-GAGUa-- -3' miRNA: 3'- uUUGGCGa-----CCGACCuCUGgCUCAaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 132403 | 0.68 | 0.839342 |
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Target: 5'- -cGCCGCgccgGGCcGGGGGCCG-GUg-- -3' miRNA: 3'- uuUGGCGa---CCGaCCUCUGGCuCAaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 134023 | 0.73 | 0.537749 |
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Target: 5'- -cGCCGCUGGUgcUGGAGGCCGcGg--- -3' miRNA: 3'- uuUGGCGACCG--ACCUCUGGCuCaaag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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