Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6370 | 3' | -54.6 | NC_001847.1 | + | 34810 | 0.7 | 0.715821 |
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Target: 5'- cGGCCGCUGGCgcGGcAGGCCGAc---- -3' miRNA: 3'- uUUGGCGACCGa-CC-UCUGGCUcaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 15391 | 0.73 | 0.568796 |
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Target: 5'- --uCgGCgucggGGCUGG-GGCCGGGUUUCg -3' miRNA: 3'- uuuGgCGa----CCGACCuCUGGCUCAAAG- -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 134023 | 0.73 | 0.537749 |
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Target: 5'- -cGCCGCUGGUgcUGGAGGCCGcGg--- -3' miRNA: 3'- uuUGGCGACCG--ACCUCUGGCuCaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 31210 | 0.73 | 0.537749 |
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Target: 5'- -cGCCGCUGGUgcUGGAGGCCGcGg--- -3' miRNA: 3'- uuUGGCGACCG--ACCUCUGGCuCaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 33859 | 0.74 | 0.467914 |
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Target: 5'- gAGACCGCgGGCUGGGGGgCGGGc--- -3' miRNA: 3'- -UUUGGCGaCCGACCUCUgGCUCaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 102412 | 0.77 | 0.352183 |
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Target: 5'- uAGCCGCcGGCcGGAGGCCGAGa--- -3' miRNA: 3'- uUUGGCGaCCGaCCUCUGGCUCaaag -5' |
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| 6370 | 3' | -54.6 | NC_001847.1 | + | 111783 | 1.06 | 0.004226 |
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Target: 5'- cAAACCGCUGGCUGGAGACCGAGUUUCc -3' miRNA: 3'- -UUUGGCGACCGACCUCUGGCUCAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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