Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6371 | 5' | -52.3 | NC_001847.1 | + | 101162 | 0.66 | 0.974333 |
Target: 5'- --cCG-GGGCCGGGGCCggGGUCGGg-- -3' miRNA: 3'- caaGCaCCUGGUCUCGG--UCAGUUaca -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 2881 | 0.66 | 0.972659 |
Target: 5'- -gUCGUcGGGCCGGgugcgucgccguucgGGCCggacGGUCGGUGUc -3' miRNA: 3'- caAGCA-CCUGGUC---------------UCGG----UCAGUUACA- -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 111327 | 0.66 | 0.961661 |
Target: 5'- --gCGcUGGACCGcGAGCaguGGUCGGUGg -3' miRNA: 3'- caaGC-ACCUGGU-CUCGg--UCAGUUACa -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 70417 | 0.68 | 0.930186 |
Target: 5'- -cUCGUGGGCUgcacGGCCGcGUCGGUGg -3' miRNA: 3'- caAGCACCUGGuc--UCGGU-CAGUUACa -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 95162 | 0.68 | 0.930186 |
Target: 5'- gGUUCGUcaGGGCgCAGAGCgAGaccUCGGUGg -3' miRNA: 3'- -CAAGCA--CCUG-GUCUCGgUC---AGUUACa -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 96140 | 0.68 | 0.924665 |
Target: 5'- -aUCG-GGGCCGGAGCCGGa------ -3' miRNA: 3'- caAGCaCCUGGUCUCGGUCaguuaca -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 45743 | 0.69 | 0.906545 |
Target: 5'- -----cGGGCCAGAGCCcGUCcGUGa -3' miRNA: 3'- caagcaCCUGGUCUCGGuCAGuUACa -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 117199 | 0.69 | 0.899992 |
Target: 5'- --cCGUGGggacGCCGGAGCCGccGUCcGUGUc -3' miRNA: 3'- caaGCACC----UGGUCUCGGU--CAGuUACA- -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 115439 | 0.71 | 0.8124 |
Target: 5'- -gUCGcgGGGCCGGAGCCGGcauUCAAa-- -3' miRNA: 3'- caAGCa-CCUGGUCUCGGUC---AGUUaca -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 109469 | 0.71 | 0.793842 |
Target: 5'- -gUCGUGGAUCgcaugAGAGCgCAGUCGcgGg -3' miRNA: 3'- caAGCACCUGG-----UCUCG-GUCAGUuaCa -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 96104 | 0.71 | 0.793842 |
Target: 5'- -gUCG-GGGCCGGAGCCggAGUCGGg-- -3' miRNA: 3'- caAGCaCCUGGUCUCGG--UCAGUUaca -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 92448 | 0.76 | 0.544842 |
Target: 5'- -aUCGUGcGACacacggcgCAGGGCCGGUCGAUGg -3' miRNA: 3'- caAGCAC-CUG--------GUCUCGGUCAGUUACa -5' |
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6371 | 5' | -52.3 | NC_001847.1 | + | 111460 | 1.07 | 0.006543 |
Target: 5'- cGUUCGUGGACCAGAGCCAGUCAAUGUc -3' miRNA: 3'- -CAAGCACCUGGUCUCGGUCAGUUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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