Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6374 | 5' | -48.4 | NC_001847.1 | + | 128719 | 0.7 | 0.97741 |
Target: 5'- gGUGC-GggGUGCGAgggggaGGGGGUGgugGCCg -3' miRNA: 3'- -CGCGaCuuCAUGCU------UCUCUACa--UGGa -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 83952 | 0.7 | 0.974774 |
Target: 5'- cGCGC-GggGccggggGCGGAGAcGUGUACCg -3' miRNA: 3'- -CGCGaCuuCa-----UGCUUCUcUACAUGGa -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 59550 | 0.7 | 0.971921 |
Target: 5'- gGCGgUGggGgcgGCGggG-GcgGUGCCg -3' miRNA: 3'- -CGCgACuuCa--UGCuuCuCuaCAUGGa -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 92034 | 0.71 | 0.968842 |
Target: 5'- cCGCUccacgucaucGgcGUACGuGGGGAUGUACCg -3' miRNA: 3'- cGCGA----------CuuCAUGCuUCUCUACAUGGa -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 36142 | 0.73 | 0.918408 |
Target: 5'- aGCGCUGuGGUACGAGgacaccggcGAGAcGUACUg -3' miRNA: 3'- -CGCGACuUCAUGCUU---------CUCUaCAUGGa -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 12984 | 0.73 | 0.918408 |
Target: 5'- gGCGCUGcAGUAcCGGcuGGGGGUGggggGCCUg -3' miRNA: 3'- -CGCGACuUCAU-GCU--UCUCUACa---UGGA- -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 50643 | 0.73 | 0.91597 |
Target: 5'- cGCGCUGAAGUAUGuggccagcacgcuGGAGGgcgacGUGCCc -3' miRNA: 3'- -CGCGACUUCAUGCu------------UCUCUa----CAUGGa -5' |
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6374 | 5' | -48.4 | NC_001847.1 | + | 109714 | 1.11 | 0.009926 |
Target: 5'- gGCGCUGAAGUACGAAGAGAUGUACCUg -3' miRNA: 3'- -CGCGACUUCAUGCUUCUCUACAUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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