Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6376 | 3' | -54 | NC_001847.1 | + | 66864 | 0.76 | 0.471426 |
Target: 5'- cGCCUcucGGgCUUCGUGUACGCGCccaaccgggAGUGCu -3' miRNA: 3'- -UGGA---CCaGAAGCACAUGUGCG---------UCGCG- -5' |
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6376 | 3' | -54 | NC_001847.1 | + | 78665 | 0.77 | 0.415889 |
Target: 5'- cGCCUGcGUCgccggCGUGcGCACGCgGGCGCu -3' miRNA: 3'- -UGGAC-CAGaa---GCACaUGUGCG-UCGCG- -5' |
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6376 | 3' | -54 | NC_001847.1 | + | 128207 | 0.85 | 0.154451 |
Target: 5'- cGCCUGGcccgUCUUCGUGUGCACcuucaaCAGCGCg -3' miRNA: 3'- -UGGACC----AGAAGCACAUGUGc-----GUCGCG- -5' |
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6376 | 3' | -54 | NC_001847.1 | + | 108490 | 1.11 | 0.002803 |
Target: 5'- uACCUGGUCUUCGUGUACACGCAGCGCu -3' miRNA: 3'- -UGGACCAGAAGCACAUGUGCGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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