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miRNA: 3'- -UGGa---CCAGaaGCAcAUGu------GCGUCGCG- -5'
miRNA: 3'- -UGGACCAGAagcacAUGUGCG-----UCGCG- -5'
miRNA: 3'- uggaCCAGaaGCACa---------UGUGCGUCGCG- -5'
miRNA: 3'- uGGACC---AGaAGCAcaUGUGCGUCGCg -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 6376 | 3' | -54 | NC_001847.1 | + | 16289 | 0.66 | 0.95222 |
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Target: 5'- cGCCggcgGGUCgaCGUaUACcgucaccCGCGGCGCg -3' miRNA: 3'- -UGGa---CCAGaaGCAcAUGu------GCGUCGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 29413 | 0.66 | 0.933763 |
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Target: 5'- aACCUGGUUUgacc-UGCGCGCcagcgAGCGCu -3' miRNA: 3'- -UGGACCAGAagcacAUGUGCG-----UCGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 47857 | 0.66 | 0.933763 |
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Target: 5'- ---aGGUCg-CGUGcugcgcgggcGCGCGCAGCGCg -3' miRNA: 3'- uggaCCAGaaGCACa---------UGUGCGUCGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 54036 | 0.66 | 0.95222 |
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Target: 5'- uUCUGGaugUCcUCGUcgGCGCGCGGCGa -3' miRNA: 3'- uGGACC---AGaAGCAcaUGUGCGUCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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