miRNA: 3'- -UGG-ACC-AGA-AGCacaUGUGCGUCGCG- -5'
miRNA: 3'- -UG-GACCA----GAagCACAUGUGCGUcGCG- -5'
miRNA: 3'- uGGACCAGaaGCACAUGUGCGUcGCg -5'
miRNA: 3'- uGGA-CCAGA---AGCa------CA---UGUGCGUCGCG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6376 | 3' | -54 | NC_001847.1 | + | 131101 | 0.74 | 0.56102 |
|
Target: 5'- cACCgUGGaUCUcUCGgg-GCGCGCGGCGCg -3' miRNA: 3'- -UGG-ACC-AGA-AGCacaUGUGCGUCGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 131504 | 0.67 | 0.911341 |
|
Target: 5'- gGCgCUGGUggacCUg-GUGUACGCGCGcCGCg -3' miRNA: 3'- -UG-GACCA----GAagCACAUGUGCGUcGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 133456 | 0.71 | 0.756438 |
|
Target: 5'- cCCUGGUUccCGUGUACAUGCA-CGg -3' miRNA: 3'- uGGACCAGaaGCACAUGUGCGUcGCg -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 134746 | 0.73 | 0.602424 |
|
Target: 5'- cCCUcGGUCUcggUCGgagcgcgGUccgGCGCGCGGCGCg -3' miRNA: 3'- uGGA-CCAGA---AGCa------CA---UGUGCGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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