miRNA: 3'- -UGGa--------CCAGa---AGCA-CAUGUgCGUCGCG- -5'
miRNA: 3'- -UG-GACCa--Gaa-GCACA---------------UGUGCGUCGCg -5'
miRNA: 3'- -UGGa------CCAGAAGCaCAUGUGcgUCGCG- -5'
miRNA: 3'- uGGACCAGaaGCA----CAuGUGCG--UCGCG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6376 | 3' | -54 | NC_001847.1 | + | 3056 | 0.66 | 0.938746 |
|
Target: 5'- aGCCgcaggcgcaGGUUgugcUCGUaGUGCA-GCAGCGCg -3' miRNA: 3'- -UGGa--------CCAGa---AGCA-CAUGUgCGUCGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 2237 | 0.68 | 0.887796 |
|
Target: 5'- gGCgCUGGgcgCgggCGUGUgguaguccccgggcgGCACGCGGCGg -3' miRNA: 3'- -UG-GACCa--Gaa-GCACA---------------UGUGCGUCGCg -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 2049 | 0.67 | 0.911341 |
|
Target: 5'- gGCCacgauagGGUCUUUG-GUGCGCa-GGCGCa -3' miRNA: 3'- -UGGa------CCAGAAGCaCAUGUGcgUCGCG- -5' |
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| 6376 | 3' | -54 | NC_001847.1 | + | 1069 | 0.69 | 0.830133 |
|
Target: 5'- cCCUGGUCcgCGUcccaGUcCACGCcgGGCGCc -3' miRNA: 3'- uGGACCAGaaGCA----CAuGUGCG--UCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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