miRNA: 3'- cAugGAUUu---GUUGAGC------AACGACGuCG- -5'
miRNA: 3'- -CAUGga--UUUGUUgaGCa---ACGACGUCG- -5'
miRNA: 3'- -CAUGGAUUUGuugaGCaaCGACguCG- -5'
miRNA: 3'- cAUGGauuUgUUGAGCa-ACG-ACGUCG- -5'
miRNA: 3'- -CAUGGAUUUGUUGAGCAACGACGUCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 638 | 5' | -47.1 | AC_000017.1 | + | 12991 | 0.67 | 0.936 |
|
Target: 5'- cUugCUGAcgccCAACUCGcgccugUUGCUGCuGCu -3' miRNA: 3'- cAugGAUUu---GUUGAGC------AACGACGuCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 33244 | 0.67 | 0.936 |
|
Target: 5'- cGUGCaucaGGAUAGggCGguggUGCUGCAGCa -3' miRNA: 3'- -CAUGga--UUUGUUgaGCa---ACGACGUCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 18907 | 0.67 | 0.941649 |
|
Target: 5'- aGUACCUGAGCcc--CGg-GCUGguGCa -3' miRNA: 3'- -CAUGGAUUUGuugaGCaaCGACguCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 25108 | 0.67 | 0.941649 |
|
Target: 5'- uUACCgu-GgAGCUUGagUGCaUGCAGCg -3' miRNA: 3'- cAUGGauuUgUUGAGCa-ACG-ACGUCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 13779 | 1.13 | 0.002172 |
|
Target: 5'- aGUACCUAAACAACUCGUUGCUGCAGCc -3' miRNA: 3'- -CAUGGAUUUGUUGAGCAACGACGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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