miRNA: 3'- -CAUGGAUUUGUu-GagCA--ACGa-CGUCG- -5'
miRNA: 3'- caUGGaUUUGUUGAGCAa----------CGACGUCG- -5'
miRNA: 3'- caUGGAUUUGuUGa--G-CAACGACGuCG- -5'
miRNA: 3'- caUGGAUUUGUUGAGCAACgaCGU---CG- -5'
miRNA: 3'- -CAUGGAUUUGUUGAGCAACGACGUCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 638 | 5' | -47.1 | AC_000017.1 | + | 10443 | 0.7 | 0.822761 |
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Target: 5'- uGUACCUGGACAucCagGUgaUGCcgGCGGCg -3' miRNA: 3'- -CAUGGAUUUGUu-GagCA--ACGa-CGUCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 13653 | 0.71 | 0.758018 |
|
Target: 5'- -gGCC-AAGCAGCUUGUccgaucuaggcGCUGCGGCc -3' miRNA: 3'- caUGGaUUUGUUGAGCAa----------CGACGUCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 11417 | 0.72 | 0.699611 |
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Target: 5'- -gGCCUGAACcGCgagCgGUUGCUGC-GCg -3' miRNA: 3'- caUGGAUUUGuUGa--G-CAACGACGuCG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 14911 | 0.76 | 0.473583 |
|
Target: 5'- -cGCCUGAGCAACUUGUUGg-GCAuccGCa -3' miRNA: 3'- caUGGAUUUGUUGAGCAACgaCGU---CG- -5' |
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| 638 | 5' | -47.1 | AC_000017.1 | + | 13779 | 1.13 | 0.002172 |
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Target: 5'- aGUACCUAAACAACUCGUUGCUGCAGCc -3' miRNA: 3'- -CAUGGAUUUGUUGAGCAACGACGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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