Results 101 - 106 of 106 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6380 | 3' | -53.9 | NC_001847.1 | + | 130619 | 0.66 | 0.946683 |
Target: 5'- gGGcCaCCGAggGCGCCgaGGCCGaagacgcgGAGAUCGg -3' miRNA: 3'- -CCaG-GGCU--UGCGG--CCGGUa-------CUUUAGC- -5' |
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6380 | 3' | -53.9 | NC_001847.1 | + | 132279 | 0.69 | 0.883854 |
Target: 5'- cGG-CCCGcGCGUCGGCC-UGGGcgCu -3' miRNA: 3'- -CCaGGGCuUGCGGCCGGuACUUuaGc -5' |
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6380 | 3' | -53.9 | NC_001847.1 | + | 133026 | 0.72 | 0.707183 |
Target: 5'- --cCCCGAgGCGCUGGCC--GAGAUCGc -3' miRNA: 3'- ccaGGGCU-UGCGGCCGGuaCUUUAGC- -5' |
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6380 | 3' | -53.9 | NC_001847.1 | + | 134185 | 0.69 | 0.846039 |
Target: 5'- cGG-CCCGggUGCCGGCgCA----GUCGu -3' miRNA: 3'- -CCaGGGCuuGCGGCCG-GUacuuUAGC- -5' |
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6380 | 3' | -53.9 | NC_001847.1 | + | 134206 | 0.67 | 0.937432 |
Target: 5'- nGUcCCCGGGCGCgGGCUcgGGcuUCc -3' miRNA: 3'- cCA-GGGCUUGCGgCCGGuaCUuuAGc -5' |
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6380 | 3' | -53.9 | NC_001847.1 | + | 134893 | 0.67 | 0.92174 |
Target: 5'- cGGgccCCUGGGCGCCGGgCGUcgGGGcgCGa -3' miRNA: 3'- -CCa--GGGCUUGCGGCCgGUA--CUUuaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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