miRNA: 3'- uaCUCAUCUAC-----CUUCUGCAU--AC-GCGU- -5'
miRNA: 3'- uACUCAUCUAcCUUCUGCauaCGCGu -5'
miRNA: 3'- -UACUCaUCUACCUU-----CUGCAUaCGCGU- -5'
miRNA: 3'- -UACUCaUCUACCUU-----CUGCAUaCGCGU- -5'
miRNA: 3'- -UACUCAUCUACCUUCUGCAUACGCGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 639 | 3' | -49.7 | AC_000017.1 | + | 6708 | 0.68 | 0.75889 |
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Target: 5'- -gGGGUGGGUGagcgcGGAGGCGUAcaUGcCGCAg -3' miRNA: 3'- uaCUCAUCUAC-----CUUCUGCAU--AC-GCGU- -5' |
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| 639 | 3' | -49.7 | AC_000017.1 | + | 28293 | 0.68 | 0.736244 |
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Target: 5'- uUGGGUGGGU-GAAGACGgugGCGUu -3' miRNA: 3'- uACUCAUCUAcCUUCUGCauaCGCGu -5' |
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| 639 | 3' | -49.7 | AC_000017.1 | + | 45 | 0.72 | 0.500652 |
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Target: 5'- aAUGAGgGGGUGGAGuuuguGACGUG-GCGCGg -3' miRNA: 3'- -UACUCaUCUACCUU-----CUGCAUaCGCGU- -5' |
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| 639 | 3' | -49.7 | AC_000017.1 | + | 35957 | 0.72 | 0.500652 |
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Target: 5'- aAUGAGgGGGUGGAGuuuguGACGUG-GCGCGg -3' miRNA: 3'- -UACUCaUCUACCUU-----CUGCAUaCGCGU- -5' |
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| 639 | 3' | -49.7 | AC_000017.1 | + | 13870 | 1.08 | 0.002238 |
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Target: 5'- gAUGAGUAGAUGGAAGACGUAUGCGCAg -3' miRNA: 3'- -UACUCAUCUACCUUCUGCAUACGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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