Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
640 | 5' | -51.1 | AC_000017.1 | + | 2870 | 0.66 | 0.814747 |
Target: 5'- uUCGAGGcuguGCCUUuuacugcugcuGGAAGggGgUGGUGu -3' miRNA: 3'- -AGCUCUu---CGGAG-----------UCUUCuuUgGCCAC- -5' |
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640 | 5' | -51.1 | AC_000017.1 | + | 24240 | 0.66 | 0.804706 |
Target: 5'- gCGGGGGuGCCUCGacGggGAAGgUGGUa -3' miRNA: 3'- aGCUCUU-CGGAGU--CuuCUUUgGCCAc -5' |
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640 | 5' | -51.1 | AC_000017.1 | + | 538 | 0.69 | 0.637092 |
Target: 5'- -aGAGuGGCCUCuuGAGGAAcucACCGGg- -3' miRNA: 3'- agCUCuUCGGAGu-CUUCUU---UGGCCac -5' |
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640 | 5' | -51.1 | AC_000017.1 | + | 15267 | 1.1 | 0.001315 |
Target: 5'- gUCGAGAAGCCUCAGAAGAAACCGGUGa -3' miRNA: 3'- -AGCUCUUCGGAGUCUUCUUUGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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