Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6411 | 5' | -52.7 | NC_001847.1 | + | 14457 | 0.66 | 0.96898 |
Target: 5'- cGgcCGCGCUGcucggCGUGcgcuccaacgccGUCCCGGAGGc -3' miRNA: 3'- -CauGCGCGACaa---GUAC------------CAGGGCUUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 35672 | 0.66 | 0.965775 |
Target: 5'- --uCGCGCUGaUCuUGGcCCCGAGc- -3' miRNA: 3'- cauGCGCGACaAGuACCaGGGCUUcu -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 89041 | 0.66 | 0.958682 |
Target: 5'- uGUGCGCGCUGggcgacggCAUGGcgCgCCGGGu- -3' miRNA: 3'- -CAUGCGCGACaa------GUACCa-G-GGCUUcu -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 37729 | 0.66 | 0.958682 |
Target: 5'- -gGCGCGCcgGUugUCGUGG-CCaUGGAGAa -3' miRNA: 3'- caUGCGCGa-CA--AGUACCaGG-GCUUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 70304 | 0.66 | 0.958682 |
Target: 5'- -gGCGCGCcGcgC-UGGUCUCGGAGc -3' miRNA: 3'- caUGCGCGaCaaGuACCAGGGCUUCu -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 128357 | 0.66 | 0.958682 |
Target: 5'- -cACGCGCUGcUgGUGG-CCaCGGAGc -3' miRNA: 3'- caUGCGCGACaAgUACCaGG-GCUUCu -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 79259 | 0.67 | 0.954782 |
Target: 5'- cGUACGUGCUGcccgcccaggUCGUGGUCaCGAu-- -3' miRNA: 3'- -CAUGCGCGACa---------AGUACCAGgGCUucu -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 93683 | 0.67 | 0.946702 |
Target: 5'- -cGCGCGUgcagcucucguuccgGUUCAUGGgcCCCGgcGAc -3' miRNA: 3'- caUGCGCGa--------------CAAGUACCa-GGGCuuCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 94802 | 0.67 | 0.946252 |
Target: 5'- -cACGCGCac-UCAaGGUCgCCGAGGGc -3' miRNA: 3'- caUGCGCGacaAGUaCCAG-GGCUUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 116733 | 0.67 | 0.936726 |
Target: 5'- -cGCGCGCUGUUCGacagccccgcgGG-CCCGcAGGc -3' miRNA: 3'- caUGCGCGACAAGUa----------CCaGGGCuUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 31122 | 0.68 | 0.926187 |
Target: 5'- -cGCGCGCUGgagC-UGGcgCCCGAcGAg -3' miRNA: 3'- caUGCGCGACaa-GuACCa-GGGCUuCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 133935 | 0.68 | 0.926187 |
Target: 5'- -cGCGCGCUGgagC-UGGcgCCCGAcGAg -3' miRNA: 3'- caUGCGCGACaa-GuACCa-GGGCUuCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 30660 | 0.69 | 0.902062 |
Target: 5'- gGUGCugGCGCUGUUgGaGGgcuaCCCGGAGGu -3' miRNA: 3'- -CAUG--CGCGACAAgUaCCa---GGGCUUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 29495 | 0.69 | 0.881358 |
Target: 5'- -cGCGCGCUGUUCAg---CCCGccGGc -3' miRNA: 3'- caUGCGCGACAAGUaccaGGGCuuCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 132308 | 0.69 | 0.881358 |
Target: 5'- -cGCGCGCUGUUCAg---CCCGccGGc -3' miRNA: 3'- caUGCGCGACAAGUaccaGGGCuuCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 66883 | 0.69 | 0.873979 |
Target: 5'- -cGCGCGCggGUacUCGUcGGUCCCGGu-- -3' miRNA: 3'- caUGCGCGa-CA--AGUA-CCAGGGCUucu -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 61754 | 0.7 | 0.866369 |
Target: 5'- -aACGUGCgauacCA-GGUCCCGAAGAc -3' miRNA: 3'- caUGCGCGacaa-GUaCCAGGGCUUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 6922 | 0.7 | 0.858534 |
Target: 5'- cUGCGCGCUGUUgAaGGUgCacaCGAAGAc -3' miRNA: 3'- cAUGCGCGACAAgUaCCA-Gg--GCUUCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 57268 | 0.7 | 0.842219 |
Target: 5'- -aGCGCGCUGUUCAaaaacUCCCGGgccgAGAg -3' miRNA: 3'- caUGCGCGACAAGUacc--AGGGCU----UCU- -5' |
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6411 | 5' | -52.7 | NC_001847.1 | + | 84347 | 1.09 | 0.005415 |
Target: 5'- uGUACGCGCUGUUCAUGGUCCCGAAGAc -3' miRNA: 3'- -CAUGCGCGACAAGUACCAGGGCUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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