Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6425 | 5' | -47.6 | NC_001847.1 | + | 32032 | 0.66 | 0.999435 |
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Target: 5'- gGCGGGgcgCGGGGAcGgcgccCGCGCGGGc -3' miRNA: 3'- -CGUUCa--GCUCCUaCaaau-GUGUGCCU- -5' |
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| 6425 | 5' | -47.6 | NC_001847.1 | + | 29832 | 0.66 | 0.999435 |
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Target: 5'- cGCAGGUCcgcaacgcgGAGGcUGUgcugggcgagcUGCACACGGu -3' miRNA: 3'- -CGUUCAG---------CUCCuACAa----------AUGUGUGCCu -5' |
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| 6425 | 5' | -47.6 | NC_001847.1 | + | 11624 | 0.71 | 0.97741 |
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Target: 5'- gGUGAGcUCGAGGAUGgggauggACACgAUGGAg -3' miRNA: 3'- -CGUUC-AGCUCCUACaaa----UGUG-UGCCU- -5' |
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| 6425 | 5' | -47.6 | NC_001847.1 | + | 3562 | 0.69 | 0.994733 |
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Target: 5'- gGCGuAGUUGAGGGUGUagcCGC-CGGGg -3' miRNA: 3'- -CGU-UCAGCUCCUACAaauGUGuGCCU- -5' |
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| 6425 | 5' | -47.6 | NC_001847.1 | + | 70 | 0.67 | 0.998935 |
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Target: 5'- gGCGGGggCGGGGugggGgaUGgGCGCGGAg -3' miRNA: 3'- -CGUUCa-GCUCCua--CaaAUgUGUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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