Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6429 | 3' | -56 | NC_001847.1 | + | 112860 | 0.66 | 0.888486 |
Target: 5'- -gUGUCGGCCUCGgcGGcGCug--CCUa -3' miRNA: 3'- aaACAGCCGGAGCa-CCuCGucuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 4275 | 0.66 | 0.888486 |
Target: 5'- ---uUCGGCCUUGaGGGGCGccgCCCc -3' miRNA: 3'- aaacAGCCGGAGCaCCUCGUcuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 8615 | 0.66 | 0.888486 |
Target: 5'- ---cUCGGCCgggggCGgggGGAGgAGcgCCCu -3' miRNA: 3'- aaacAGCCGGa----GCa--CCUCgUCuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 60085 | 0.66 | 0.888486 |
Target: 5'- -gUGaUGGCCguguUCGUGG-GCAGccUCCCg -3' miRNA: 3'- aaACaGCCGG----AGCACCuCGUCu-AGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 111428 | 0.66 | 0.888486 |
Target: 5'- ---cUCGGCCgggggCGgggGGAGgAGcgCCCu -3' miRNA: 3'- aaacAGCCGGa----GCa--CCUCgUCuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 107088 | 0.66 | 0.888486 |
Target: 5'- ---uUCGGCCUUGaGGGGCGccgCCCc -3' miRNA: 3'- aaacAGCCGGAGCaCCUCGUcuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 76388 | 0.66 | 0.888486 |
Target: 5'- ----aCGGCCUCG-GGGGgGGcgCCg -3' miRNA: 3'- aaacaGCCGGAGCaCCUCgUCuaGGg -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 99028 | 0.66 | 0.881467 |
Target: 5'- --cG-CGGCUUCGUGGAa-GGcgCCCa -3' miRNA: 3'- aaaCaGCCGGAGCACCUcgUCuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 10707 | 0.66 | 0.881467 |
Target: 5'- --cGcCGGCCUCGgaGGAGgGccUCCCg -3' miRNA: 3'- aaaCaGCCGGAGCa-CCUCgUcuAGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 51527 | 0.66 | 0.874219 |
Target: 5'- --cGcCGGCaggCG-GGGGCGGcgCCCa -3' miRNA: 3'- aaaCaGCCGga-GCaCCUCGUCuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 74291 | 0.66 | 0.874219 |
Target: 5'- --cGcUGGCCUCGgcgGGcGCGGAgcugcuguuUCCCa -3' miRNA: 3'- aaaCaGCCGGAGCa--CCuCGUCU---------AGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 99777 | 0.66 | 0.866749 |
Target: 5'- --cG-CGGCUUUGggcggGGGGCAGcgCCUg -3' miRNA: 3'- aaaCaGCCGGAGCa----CCUCGUCuaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 44760 | 0.66 | 0.866749 |
Target: 5'- --cGUaCGGCCgggggcCGUGGuccgcguccAGCAGGUCgCCg -3' miRNA: 3'- aaaCA-GCCGGa-----GCACC---------UCGUCUAG-GG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 118457 | 0.66 | 0.866749 |
Target: 5'- -gUGgCGGCCUCGUGccuGCGGcgcaaggCCCg -3' miRNA: 3'- aaACaGCCGGAGCACcu-CGUCua-----GGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 15389 | 0.67 | 0.84306 |
Target: 5'- --gGUCGGCgUCGgggcUGGGGcCGGGUUUCg -3' miRNA: 3'- aaaCAGCCGgAGC----ACCUC-GUCUAGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 13726 | 0.67 | 0.825411 |
Target: 5'- -aUGacagCGGCCUuccugggCGUGGGGCuGAUCgCg -3' miRNA: 3'- aaACa---GCCGGA-------GCACCUCGuCUAGgG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 40702 | 0.67 | 0.808754 |
Target: 5'- --aGUCGGUCUgCGUGG-GC-GAaCCCa -3' miRNA: 3'- aaaCAGCCGGA-GCACCuCGuCUaGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 31936 | 0.67 | 0.808754 |
Target: 5'- ----cCGGUCUCGgucGGAGCGcGGUCCg -3' miRNA: 3'- aaacaGCCGGAGCa--CCUCGU-CUAGGg -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 98832 | 0.68 | 0.803367 |
Target: 5'- --aGUaGGCUUCGUagaGGcgcauccgcagcaccAGCAGAUCCCg -3' miRNA: 3'- aaaCAgCCGGAGCA---CC---------------UCGUCUAGGG- -5' |
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6429 | 3' | -56 | NC_001847.1 | + | 57930 | 0.68 | 0.799744 |
Target: 5'- gUUUGacgCGGCCaaacUGGAGCAGG-CCCg -3' miRNA: 3'- -AAACa--GCCGGagc-ACCUCGUCUaGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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