Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 643 | 3' | -51.3 | AC_000017.1 | + | 21374 | 0.66 | 0.814747 |
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Target: 5'- gGugGa--GGAUGCCCACCugUuGGUAGu -3' miRNA: 3'- -CugCaagUCUAUGGGUGGugG-UCAUC- -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 17854 | 0.66 | 0.814747 |
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Target: 5'- cGACGgugCGacgcGCgCCGCCGCCGGUGGu -3' miRNA: 3'- -CUGCaa-GUcua-UG-GGUGGUGGUCAUC- -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 22102 | 0.67 | 0.729444 |
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Target: 5'- aGCGUgCAGggGCCCaacucgGCCGCCuGUGGc -3' miRNA: 3'- cUGCAaGUCuaUGGG------UGGUGGuCAUC- -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 8172 | 0.67 | 0.729444 |
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Target: 5'- gGAUGUaCAGcccGUGCaCCGCUGCCAGUAc -3' miRNA: 3'- -CUGCAaGUC---UAUG-GGUGGUGGUCAUc -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 12258 | 0.68 | 0.67211 |
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Target: 5'- cGCGcgCGGGUG-CCACCGCCAGc-- -3' miRNA: 3'- cUGCaaGUCUAUgGGUGGUGGUCauc -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 26660 | 0.7 | 0.590379 |
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Target: 5'- uGGCGUUCuuGUGCCCgcGCCACgagCGGUAGc -3' miRNA: 3'- -CUGCAAGucUAUGGG--UGGUG---GUCAUC- -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 25609 | 0.77 | 0.227552 |
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Target: 5'- aGCGUUCAGGaauccUGCCCGCCACCugcuGUGc -3' miRNA: 3'- cUGCAAGUCU-----AUGGGUGGUGGu---CAUc -5' |
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| 643 | 3' | -51.3 | AC_000017.1 | + | 17193 | 1.08 | 0.001695 |
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Target: 5'- gGACGUUCAGAUACCCACCACCAGUAGc -3' miRNA: 3'- -CUGCAAGUCUAUGGGUGGUGGUCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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