Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 643 | 5' | -58.1 | AC_000017.1 | + | 23587 | 0.66 | 0.395132 |
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Target: 5'- cGCUgaguCUGCCgaucgugucugcgugGgAGaAGGGCAUGGAGGc -3' miRNA: 3'- aCGGu---GACGG---------------UgUC-UCCCGUACCUCU- -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 30516 | 0.66 | 0.391401 |
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Target: 5'- aGCguCUGCUACAGAGGcGCAaagcagcGGcAGAg -3' miRNA: 3'- aCGguGACGGUGUCUCC-CGUa------CC-UCU- -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 9085 | 0.67 | 0.35538 |
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Target: 5'- cGCCcgcgaUGCCGaAGGGGGCGUGGu-- -3' miRNA: 3'- aCGGug---ACGGUgUCUCCCGUACCucu -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 15160 | 0.67 | 0.325011 |
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Target: 5'- aUGCCAUucgcggcgacaccuuUGCCACAc-GGGCGgaGGAGAa -3' miRNA: 3'- -ACGGUG---------------ACGGUGUcuCCCGUa-CCUCU- -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 23918 | 0.68 | 0.298139 |
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Target: 5'- cGCCGCcucaGCCGCuuuuuuGGGGGCGcgcgGGGGGc -3' miRNA: 3'- aCGGUGa---CGGUGu-----CUCCCGUa---CCUCU- -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 26805 | 0.7 | 0.217086 |
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Target: 5'- cUGCCGCUGCCgccgguGCAGuaGGGGCuguaGAGAu -3' miRNA: 3'- -ACGGUGACGG------UGUC--UCCCGuac-CUCU- -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 5531 | 0.71 | 0.189285 |
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Target: 5'- gGCCA-UGCCGCGGAGGGgcUGGAc- -3' miRNA: 3'- aCGGUgACGGUGUCUCCCguACCUcu -5' |
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| 643 | 5' | -58.1 | AC_000017.1 | + | 17228 | 1.08 | 0.000264 |
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Target: 5'- uUGCCACUGCCACAGAGGGCAUGGAGAc -3' miRNA: 3'- -ACGGUGACGGUGUCUCCCGUACCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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